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jo urn al pr e p roo f igg2b monoclonal  (R&D Systems)


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    R&D Systems jo urn al pr e p roo f igg2b monoclonal
    Jo Urn Al Pr E P Roo F Igg2b Monoclonal, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+igg+isotype+control+antibody/Rat+IgG2B+Isotype+Control/pm41999979-580-0-9
    Average 93 stars, based on 153 article reviews
    jo urn al pr e p roo f igg2b monoclonal - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Neutralization:

    Article Title: Keratin 17 Promotes T Cell Response in Allergic Contact Dermatitis by Upregulating C–C Motif Chemokine Ligand 20
    Article Snippet: .. For neutralization of the CCL20 experiment, C57BL/6J mice were intraperitoneally (i.p.) injected with CCL20-neutralizing monoclonal antibody (CCL20 mAb, 100 μg/mouse, R&D Systems, USA), or rat IgG isotype control antibody (100 μg/mouse, R&D Systems, USA). ..

    Injection:

    Article Title: Keratin 17 Promotes T Cell Response in Allergic Contact Dermatitis by Upregulating C–C Motif Chemokine Ligand 20
    Article Snippet: .. For neutralization of the CCL20 experiment, C57BL/6J mice were intraperitoneally (i.p.) injected with CCL20-neutralizing monoclonal antibody (CCL20 mAb, 100 μg/mouse, R&D Systems, USA), or rat IgG isotype control antibody (100 μg/mouse, R&D Systems, USA). ..

    Article Title: Dissecting the immune suppressive human prostate tumor microenvironment via integrated single-cell and spatial transcriptomic analyses
    Article Snippet: RM1 prostate cancer cells (0.25 × 10 6 cells) were injected subcutaneously to C57BL6/J male mice (#000664) from The Jackson Laboratory. .. When tumor reached the volume of 300–400 mm 3 , mice received an intraperitoneal injection of 45 μg anti-CCL20-blocking antibody (R&D Systems, clone 114908) or rat IgG isotype control antibody (R&D Systems, clone 43414), 150 μg of anti-mouse PD-1 (BioXcell, clone RMP1-14) or rat IgG2a isotype control (BioXcell, clone 2A3). ..

    Article Title: Human prostate cancer bone metastases have an actionable immunosuppressive microenvironment
    Article Snippet: .. C57BL6/J male mice (#000664) received an intraperitoneal injection of 45μg anti-CCL20 blocking antibody (R&D Systems clone 114908) or rat IgG isotype control antibody (R&D Systems, clone 43414) 1 day prior to intracardiac injection of RM1-BoM3 prostate cancer bone metastasis cells. ..

    Article Title: Human prostate cancer bone metastases have an actionable immunosuppressive microenvironment.
    Article Snippet: CCL20 blocking antibody treatment C57BL6/J male mice (#000664) received an intraperitoneal injection of 45mg anti-CCL20 blocking antibody (R&D Systems clone 114908) or rat IgG isotype control antibody (R&D Systems, clone 43414) 1 day prior to intracardiac injection of RM1-BoM3 PCa bone metastases cells. .. CCL20 blocking antibody treatment C57BL6/J male mice (#000664) received an intraperitoneal injection of 45mg anti-CCL20 blocking antibody (R&D Systems clone 114908) or rat IgG isotype control antibody (R&D Systems, clone 43414) 1 day prior to intracardiac injection of RM1-BoM3 PCa bone metastases cells. .. In vitro stimulation of CD8+ CTLs Sorted CD8+CTLswere plated in 96-well plates in RPMI 1640 (Corning) supplemented with 10%FBS (Gibco), 10mMHEPEs (Gibco), 1% Penicillin/streptomycin (Gibco), 1% Non-essential amino acids (Gibco) and 50mM ß-mercaptoethanol.

    Article Title: Interferon-λ3 Promotes Epithelial Defense and Barrier Function Against Cryptosporidium parvum Infection.
    Article Snippet: .. Each mouse pup was administered either 15 mg of IFN-l2/3 neutralizing antibody (Monoclonal Rat IgG2B clone 244716, catalog MAB17892; R&D Systems, Minneapolis, MN) or 15 mg of rat IgG isotype control antibody (Monoclonal Rat IgG2B clone 141945, catalog MAB0061; R&D Systems) by intraperitoneal injection on days -1, 0, and 3 of infection. ..

    Article Title: Dissecting the immune suppressive human prostate tumor microenvironment via integrated single-cell and spatial transcriptomic analyses.
    Article Snippet: RM1 prostate cancer cells (0.25 × 106 cells) were injected subcutaneously to C57BL6/J male mice (#000664) from The Jackson Laboratory. .. When tumor reached the volume of 300–400mm3, mice received an intraperitoneal injection of 45μg anti-CCL20-blocking antibody (R&D Systems, clone 114908) or rat IgG isotype control antibody (R&D Systems, clone 43414), 150μg of anti-mouse PD-1 (BioXcell, clone RMP1-14) or rat IgG2a isotype control (BioXcell, clone 2A3). ..

    Control:

    Article Title: Keratin 17 Promotes T Cell Response in Allergic Contact Dermatitis by Upregulating C–C Motif Chemokine Ligand 20
    Article Snippet: .. For neutralization of the CCL20 experiment, C57BL/6J mice were intraperitoneally (i.p.) injected with CCL20-neutralizing monoclonal antibody (CCL20 mAb, 100 μg/mouse, R&D Systems, USA), or rat IgG isotype control antibody (100 μg/mouse, R&D Systems, USA). ..

    Article Title: Anti-CD3 antibody and molecules comprising the antibody
    Article Snippet: Human T cell line Jurkat cells (ATCC, No. TIB-152) were adjusted to a concentration of 5×106 cells/mL in an RPMI1640 medium containing FBS and added at a concentration of 100 μL/well to a 96-well plate. .. After removal of the supernatant by centrifugation, the culture supernatant of each anti-human CD3 antibody production-positive hybridoma selected by Cell-ELISA in Example 1)-4 or a rat IgG isotype control antibody (R&D Systems, Inc.) was added at a final concentration of 5 μg/mL to the Jurkat cells, and the plate was left standing at 37° C. for 30 minutes. .. Then, the cross-linker Goat Anti-rat IgG Fcγ Fragment specific (Jackson ImmunoResearch Laboratories, Inc.) was added at a final concentration of 10 μg/well, and the cells were cultured overnight at 37° C. under 5% CO2 conditions.

    Article Title: Dissecting the immune suppressive human prostate tumor microenvironment via integrated single-cell and spatial transcriptomic analyses
    Article Snippet: RM1 prostate cancer cells (0.25 × 10 6 cells) were injected subcutaneously to C57BL6/J male mice (#000664) from The Jackson Laboratory. .. When tumor reached the volume of 300–400 mm 3 , mice received an intraperitoneal injection of 45 μg anti-CCL20-blocking antibody (R&D Systems, clone 114908) or rat IgG isotype control antibody (R&D Systems, clone 43414), 150 μg of anti-mouse PD-1 (BioXcell, clone RMP1-14) or rat IgG2a isotype control (BioXcell, clone 2A3). ..

    Article Title: Human prostate cancer bone metastases have an actionable immunosuppressive microenvironment
    Article Snippet: .. C57BL6/J male mice (#000664) received an intraperitoneal injection of 45μg anti-CCL20 blocking antibody (R&D Systems clone 114908) or rat IgG isotype control antibody (R&D Systems, clone 43414) 1 day prior to intracardiac injection of RM1-BoM3 prostate cancer bone metastasis cells. ..

    Article Title: Human prostate cancer bone metastases have an actionable immunosuppressive microenvironment.
    Article Snippet: CCL20 blocking antibody treatment C57BL6/J male mice (#000664) received an intraperitoneal injection of 45mg anti-CCL20 blocking antibody (R&D Systems clone 114908) or rat IgG isotype control antibody (R&D Systems, clone 43414) 1 day prior to intracardiac injection of RM1-BoM3 PCa bone metastases cells. .. CCL20 blocking antibody treatment C57BL6/J male mice (#000664) received an intraperitoneal injection of 45mg anti-CCL20 blocking antibody (R&D Systems clone 114908) or rat IgG isotype control antibody (R&D Systems, clone 43414) 1 day prior to intracardiac injection of RM1-BoM3 PCa bone metastases cells. .. In vitro stimulation of CD8+ CTLs Sorted CD8+CTLswere plated in 96-well plates in RPMI 1640 (Corning) supplemented with 10%FBS (Gibco), 10mMHEPEs (Gibco), 1% Penicillin/streptomycin (Gibco), 1% Non-essential amino acids (Gibco) and 50mM ß-mercaptoethanol.

    Article Title: Interferon-λ3 Promotes Epithelial Defense and Barrier Function Against Cryptosporidium parvum Infection.
    Article Snippet: .. Each mouse pup was administered either 15 mg of IFN-l2/3 neutralizing antibody (Monoclonal Rat IgG2B clone 244716, catalog MAB17892; R&D Systems, Minneapolis, MN) or 15 mg of rat IgG isotype control antibody (Monoclonal Rat IgG2B clone 141945, catalog MAB0061; R&D Systems) by intraperitoneal injection on days -1, 0, and 3 of infection. ..

    Article Title: Dissecting the immune suppressive human prostate tumor microenvironment via integrated single-cell and spatial transcriptomic analyses.
    Article Snippet: RM1 prostate cancer cells (0.25 × 106 cells) were injected subcutaneously to C57BL6/J male mice (#000664) from The Jackson Laboratory. .. When tumor reached the volume of 300–400mm3, mice received an intraperitoneal injection of 45μg anti-CCL20-blocking antibody (R&D Systems, clone 114908) or rat IgG isotype control antibody (R&D Systems, clone 43414), 150μg of anti-mouse PD-1 (BioXcell, clone RMP1-14) or rat IgG2a isotype control (BioXcell, clone 2A3). ..

    Centrifugation:

    Article Title: Anti-CD3 antibody and molecules comprising the antibody
    Article Snippet: Human T cell line Jurkat cells (ATCC, No. TIB-152) were adjusted to a concentration of 5×106 cells/mL in an RPMI1640 medium containing FBS and added at a concentration of 100 μL/well to a 96-well plate. .. After removal of the supernatant by centrifugation, the culture supernatant of each anti-human CD3 antibody production-positive hybridoma selected by Cell-ELISA in Example 1)-4 or a rat IgG isotype control antibody (R&D Systems, Inc.) was added at a final concentration of 5 μg/mL to the Jurkat cells, and the plate was left standing at 37° C. for 30 minutes. .. Then, the cross-linker Goat Anti-rat IgG Fcγ Fragment specific (Jackson ImmunoResearch Laboratories, Inc.) was added at a final concentration of 10 μg/well, and the cells were cultured overnight at 37° C. under 5% CO2 conditions.

    Concentration Assay:

    Article Title: Anti-CD3 antibody and molecules comprising the antibody
    Article Snippet: Human T cell line Jurkat cells (ATCC, No. TIB-152) were adjusted to a concentration of 5×106 cells/mL in an RPMI1640 medium containing FBS and added at a concentration of 100 μL/well to a 96-well plate. .. After removal of the supernatant by centrifugation, the culture supernatant of each anti-human CD3 antibody production-positive hybridoma selected by Cell-ELISA in Example 1)-4 or a rat IgG isotype control antibody (R&D Systems, Inc.) was added at a final concentration of 5 μg/mL to the Jurkat cells, and the plate was left standing at 37° C. for 30 minutes. .. Then, the cross-linker Goat Anti-rat IgG Fcγ Fragment specific (Jackson ImmunoResearch Laboratories, Inc.) was added at a final concentration of 10 μg/well, and the cells were cultured overnight at 37° C. under 5% CO2 conditions.

    Blocking Assay:

    Article Title: Human prostate cancer bone metastases have an actionable immunosuppressive microenvironment
    Article Snippet: .. C57BL6/J male mice (#000664) received an intraperitoneal injection of 45μg anti-CCL20 blocking antibody (R&D Systems clone 114908) or rat IgG isotype control antibody (R&D Systems, clone 43414) 1 day prior to intracardiac injection of RM1-BoM3 prostate cancer bone metastasis cells. ..

    Article Title: Human prostate cancer bone metastases have an actionable immunosuppressive microenvironment.
    Article Snippet: CCL20 blocking antibody treatment C57BL6/J male mice (#000664) received an intraperitoneal injection of 45mg anti-CCL20 blocking antibody (R&D Systems clone 114908) or rat IgG isotype control antibody (R&D Systems, clone 43414) 1 day prior to intracardiac injection of RM1-BoM3 PCa bone metastases cells. .. CCL20 blocking antibody treatment C57BL6/J male mice (#000664) received an intraperitoneal injection of 45mg anti-CCL20 blocking antibody (R&D Systems clone 114908) or rat IgG isotype control antibody (R&D Systems, clone 43414) 1 day prior to intracardiac injection of RM1-BoM3 PCa bone metastases cells. .. In vitro stimulation of CD8+ CTLs Sorted CD8+CTLswere plated in 96-well plates in RPMI 1640 (Corning) supplemented with 10%FBS (Gibco), 10mMHEPEs (Gibco), 1% Penicillin/streptomycin (Gibco), 1% Non-essential amino acids (Gibco) and 50mM ß-mercaptoethanol.

    Infection:

    Article Title: Interferon-λ3 Promotes Epithelial Defense and Barrier Function Against Cryptosporidium parvum Infection.
    Article Snippet: .. Each mouse pup was administered either 15 mg of IFN-l2/3 neutralizing antibody (Monoclonal Rat IgG2B clone 244716, catalog MAB17892; R&D Systems, Minneapolis, MN) or 15 mg of rat IgG isotype control antibody (Monoclonal Rat IgG2B clone 141945, catalog MAB0061; R&D Systems) by intraperitoneal injection on days -1, 0, and 3 of infection. ..



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    Image Search Results


    CLCA4 suppressed colorectal cancer stem cell expansion by interacting with vimentin to suppress FAK signaling pathways. (A) Western blotting analysis of FAK and p-FAK protein levels in control and CLCA4-overexpressing colorectal cancer (CRC) cells. Right panels: Quantification of protein expression ratio. (B) Western blotting analysis of stemness-related proteins and p-FAK in CLCA4-overexpressing cells treated with or without FAK agonist. Lower panels: Quantification of protein expression ratio. (C) Tumorsphere formation assay was performed to examine the tumorsphere formation ability in CLCA4-overexpressing cells treated with or without FAK agonist. One-way ANOVA with Tukey's multiple comparisons test (mean ± standard deviation). (D) Immunoprecipitation and IgG samples were analyzed by mass spectrometry. Proteins with unused >1.3 were filtered out, and keratin was removed. A total of 336 proteins were identified, including 334 proteins in immunoprecipitation samples and 4 proteins in IgG samples. (E) The immunoprecipitates of CLCA4 were purified using anti-Flag antibody and separated with SDS-PAGE, and the presence of vimentin was analyzed by Western blotting. Normal IgG was used as the negative control. (F) The immunoprecipitates of vimentin were purified using anti-HA antibody and separated with SDS-PAGE, and the presence of CLCA4 was analyzed by Western blotting. Normal IgG was used as the negative control. (G) The differences in protein levels (vimentin, Bmi-1, and p-FAK) among CRC cells transfected with different plasmids were analyzed by Western blotting. Right panels: Quantification of protein expression ratio. (H) Tumorsphere formation assay was performed to examine the tumorsphere formation ability among CRC cells transfected with different plasmids. One-way ANOVA with Tukey's multiple comparisons test (mean ± standard deviation).

    Journal: Genes & Diseases

    Article Title: Chloride channel accessory 4 suppresses stem cell-like properties of colorectal cancer and enhances anti-PD-1 immunotherapy

    doi: 10.1016/j.gendis.2025.101859

    Figure Lengend Snippet: CLCA4 suppressed colorectal cancer stem cell expansion by interacting with vimentin to suppress FAK signaling pathways. (A) Western blotting analysis of FAK and p-FAK protein levels in control and CLCA4-overexpressing colorectal cancer (CRC) cells. Right panels: Quantification of protein expression ratio. (B) Western blotting analysis of stemness-related proteins and p-FAK in CLCA4-overexpressing cells treated with or without FAK agonist. Lower panels: Quantification of protein expression ratio. (C) Tumorsphere formation assay was performed to examine the tumorsphere formation ability in CLCA4-overexpressing cells treated with or without FAK agonist. One-way ANOVA with Tukey's multiple comparisons test (mean ± standard deviation). (D) Immunoprecipitation and IgG samples were analyzed by mass spectrometry. Proteins with unused >1.3 were filtered out, and keratin was removed. A total of 336 proteins were identified, including 334 proteins in immunoprecipitation samples and 4 proteins in IgG samples. (E) The immunoprecipitates of CLCA4 were purified using anti-Flag antibody and separated with SDS-PAGE, and the presence of vimentin was analyzed by Western blotting. Normal IgG was used as the negative control. (F) The immunoprecipitates of vimentin were purified using anti-HA antibody and separated with SDS-PAGE, and the presence of CLCA4 was analyzed by Western blotting. Normal IgG was used as the negative control. (G) The differences in protein levels (vimentin, Bmi-1, and p-FAK) among CRC cells transfected with different plasmids were analyzed by Western blotting. Right panels: Quantification of protein expression ratio. (H) Tumorsphere formation assay was performed to examine the tumorsphere formation ability among CRC cells transfected with different plasmids. One-way ANOVA with Tukey's multiple comparisons test (mean ± standard deviation).

    Article Snippet: After 7 days, mice were intraperitoneally treated with either an in vivo blocking antibody against mouse PD-1 (Clone: 29F.1A2, BioXcell, Cat# BP0273) or a rat IgG2a isotype control antibody (Clone: 2A3, BioXcell, Cat# BP0089).

    Techniques: Protein-Protein interactions, Western Blot, Control, Expressing, Tube Formation Assay, Standard Deviation, Immunoprecipitation, Mass Spectrometry, Purification, SDS Page, Negative Control, Transfection